Industrial PCR Training
Syamala Srinivasa Life Sciences Pvt.Ltd is a core Life Sciences organization exploring new horizons in flied of Molecular biology, Proteomics, Bioinformatics, and Genetics. Extending its services to develop intellectual capital have initiated training programs and hands-on workshops nationwide. This shall nourish and nurture the upcoming talent to acquire the necessary practical skill set to mark an entry in the Life Sciences industry.
We aim to support the academic learning’s with practical industrial insights and wish to educate the students on latest molecular biology techniques.
Program Schedule
1st Day:
|
Topic |
08.00 am - 09.00 am |
Registration |
09.00 am - 10.00 am |
Introduction to the workshop |
10.00 am - 10.20 am |
Break |
10.20 am - 11.20 am |
An overview on PCR |
11.20 am - 12.20 pm |
Components and Conditions for PCR optimization |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 02.00 pm |
Various methods of DNA extraction |
02.00 pm - 04.00 pm |
Lab Work: Isolation of DNA from whole blood |
2nd Day:
|
Topic |
08.00 am - 09.00 am |
PCR amplification of DNA fragment |
09.00 am - 10.00 am |
Lab work: PCR and thermal cycling |
10.00 am - 10.20 am |
Break |
10.20 am - 12.20 am |
Gel electrophoresis to check PCR products |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 04.00 pm |
Lab work: UV Trans illumination using gel documentation system followed by result interpretation |
3rd Day:
|
Topic |
08.00 am - 10.00 am |
Introduction to amino acids, Proteins. Advanced Proteomic techniques and applications |
10.00 am - 10.20 am |
Break |
10.20 am - 12.20 pm |
Lab work: SDS PAGE: Gel casting, Protein sample extraction, Running gel |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 02.00 pm |
Gel staining |
02.00 pm - 04.00 pm |
Visualization of gels, Rf value calculations and interpretation. |
4th Day:
|
Topic |
08.00 am - 10.00 am |
Human genome to Omics…………… |
10.00 am - 10.20 am |
Break |
10.20 am - 12.20 am |
RNA isolation from blood |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 04.00 pm |
Lab work: RT-PCR Gel electrophoresis, UV trans illumination using documentation system and gel interpretation |
5th Day:
|
Topic |
08.00 am - 10.00 am |
Bioinformatics |
10.00 am - 10.20 am |
Break time |
10.20 am - 12.20 pm |
Biological sequence data analysis |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 04.00 pm |
Lab work: Biological Data analysis, Sequence alignment, Protein Data bank etc., |
6 th Day:
Time
|
Topic
|
08.00 am - 10.00 am |
Overview of chromatography and applications of HPLC |
10.00 am - 10.20 am |
Break time |
10.20 am - 12.20 pm |
Discussion Chromatographic terms resolution, accuracy, precision, run time |
12.20 pm - 01.00 pm |
Lunch time |
01.00 pm - 04.00 pm |
Career Prospects in Life Sciencesnology and Certificate award. |
