Projects and Trainings in Separation Biology Department
Module I: Separation techniques
Centrifugation
Separation of cell population
Electrophoresis
DNA electrophoresis
Agarose
Polyacrylamide (PAGE)
Denaturative (Formamide, urea)
Protein electrophoresis
Native PAGE
Continuous electrophoresis
Discontinuous electrophoresis (DISC)
Denaturative PAGE (SDS)
Western blotting
Chromatography
Introduction to Chromatography
Paper chromatography
Thin layer chromatography
Gel-filtration chromatography
Ion-exchange chromatography
Affinity chromatography
High Performance Liquid Chromatography
Module II: HPLC
Method development
Physicochemical characterization
Solubility studies
PKa determination
Log P
Determination of lambda max
Selection of mobile phase
Selection of column
Other parameters (temp, flow rate etc.)
Validation parameters
System suitability
Linearity and range
Dilution integrity
LLOD (Lower Limit Of Detection)
LLOQ (Lower Limit Of Quantification)
Reproducibility
Stability
Intra day
Inter day
Forced degradation
Oxidation
Reduction
Acid
Alkaline
Photolytic
Sensitivity
Accuracy and precision
Extraction recovery
Specificity
Robustness
Ruggedness
Drug testing as per Compendial methods Identification
Detection and quantification of impurities
Related substances Assay
Pharmacokinetic studies
Bioanalytical HPLC method development and validation
Determination of drug concentrations in biological fluids at different time intervals
Determination of Pharmacokinetic parameters
Concentration maximum (C max)
Time maximum (T max)
Volume of distribution (Vd)
Clearance (Cl)
Rate constant (K)
Half life (T ½)
Area under the curve (AUC)
Module III: Immunological techniques
Serum antibody isolation
Antibody Purification
Immuno diffusion (Ouchterlony)
Immuno electrophoresis
Rocket immunoelectrophoresis
Western blotting
